Integration site of human papillomavirus type-18 DNA in chromosome band 8q22.1 of C4-1 cervical carcinoma: DNase I hypersensitivity and methylation of cellular flanking sequences
✍ Scribed by Marta I. Gallego; Dr. Pedro A. Lazo; Drazen B. Zimonjic; Nicholas C. Popescu; Joseph A. Dipaolo
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 460 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
The C4-I cell line derived from a non-keratinizing squamous cell carcinoma of the uterine cervix contains integrated human papillomavirus-I8 DNA. Fluorescence in situ hybridization of C4-I cells demonstrated a single viral integration site at 8q22. I on a derivative chromosome originating from an 8q;12q translocation. 8q22 is a site of chromosome fragility and is also recombinogenic in several human malignancies. DNase I hypersensitivity of the integration site was studied with a cellular flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4-I and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells. Genes Chrom Cancer 928-32 (1994).