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Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum

✍ Scribed by Liang Gao; Jie Zhang; Weibing Zhang; Yichu Shan; Zhen Liang; Lihua Zhang; Yushu Huo; Yukui Zhang

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
153 KB
Volume
33
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

In this study, a comprehensive 2‐D chromatography was constructed, consisting of normal phase LC (NPLC) with a CN column as the first dimension, and supercritical fluid chromatography (SFC), with a Merck Chromolith Flash C~18~ column as the second dimension, which were connected by a 10‐port, dual‐position valve controlled automatically by a self‐designed software. Such platform was applied into the analysis of the fruiting bodies of Ganoderma lucidum, a traditional Chinese medicine, and within 2 h analysis, the obtained peak capacity of the 2‐D‐NPLC‐SFC system was about 350, obviously higher than that of each dimension. These results demonstrate that 2‐D‐NPLC‐SFC is not only of good orthogonality, but also of high throughput for the analysis of complex samples.

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