Integration of IS3 into IS2 generates a short sequence duplication

✍ Scribed by Sommer, Hans ;Cullum, John ;Saedler, Heinz

• Book ID: 104692880
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 418 KB
• Volume: 177
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00267256

✦ Synopsis

The Gal+ allele IS2-43 is known to segregate Gal- clones. Among 11 Gal- segregants, one was shown to be due to the integration of IS3 into IS2-43. Precise excision of the integrated IS3 element occurred at a rate of 5 x 10(-9)/cell/generation. DNA sequence analysis revealed that the termini of the IS3 element have the relation of imperfect inverted repeats and it is now flanked by a 3bp or 4bp duplication, a size which has not been seen before with other elements.