Integration of ileum cannulated pigs andin vitro fermentation to quantify the effect of diet composition on the amount of short-chain fatty acids available from fermentation in the large intestine
✍ Scribed by Christensen, Dorthe N; Knudsen, Knud Erik Bach; Wolstrup, Jens; Jensen, Bent Borg
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 146 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
✦ Synopsis
Using cannulated pigs and a standardised in vitro fermentation system the eþ ect of diet and non-starch polysaccharides (NSP) on the amount of energy available from microbial fermentation in the large intestine could be predicted. The available energy was calculated from the amounts of shortchain fatty acids (SCFA) produced. Three diets were investigated : a low übre diet based essentially on wheat ýour (56 g NSP kg-1 feed) and two high übre diets with added oat bran (93 g NSP kg-1 feed) or wheat bran (102 g NSP kg-1 feed). Colonic fermentation was estimated by in vitro fermentation of freeze-dried ileal effluent collected from cannulated pigs. The in vitro fermentation method was optimised to use 10 g ileum content litre-1 incubated at pH 6.0 in a fermentor containing faecal slurry consisting of anaerobic mineral salts medium and 50 g litre-1 faeces from pigs fed the same diets as the cannulated pigs. The results demonstrate that it is very important to compensate for the faecal SCFA contribution when calculating the amount of SCFA produced from ileal digesta during in vitro fermentation. The amount of NSP digested in vitro was compared with data obtained from in vivo studies and there was a good agreement between in vivo and in vitro data. We concluded that the integrated in vivo-in vitro method is a valuable technique to estimate the eþ ect of diet and NSP on the amount of SCFA produced in the large intestine and when fed the three diets the microbial fermentation in the large intestine provided between 2.4-6.4% of the total available energy.