Integration of cellular adhesion and Wnt signaling: Interactions between N-cadherin and LRP5 and their role in regulating bone mass

A cell-based Dkk1 binding assay reveals

A cell-based Dkk1 binding assay reveals roles for extracellular domains of LRP5 in Dkk1 interaction and highlights differences between wild-type and the high bone mass mutant LRP5(G171V)

✍ Richard J. Murrills; Jeanne J. Matteo; Bheem M. Bhat; Valerie E. Coleburn; Krist 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 276 KB 👁 2 views

## Abstract Dkk1 is a secreted antagonist of the LRP5‐mediated Wnt signaling pathway that plays a pivotal role in bone biology. Because there are no well‐documented LRP5‐based assays of Dkk1 binding, we developed a cell‐based assay of Dkk1/LRP5 binding using radioactive ^125^I‐Dkk1. In contrast to