Integration of adeno-associated virus 2 DNA in human MKR melanoma cells induces a peptide with oncosuppressive properties

✍ Scribed by Ursula Bantel-Schaal

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: French
• Weight: 495 KB
• Volume: 92
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.1230

✦ Synopsis

Integration of adeno-associated virus type 2 (AAV2) DNA into the genome of the human MKr melanoma cell line grossly alters the cellular phenotype of these cells. Cultures derived from AAV DNA-harboring single cells share various similarities with normal cells in culture, and a considerable number of clones show signs of cellular senescence and terminal differentiation. Medium conditioned by such terminally differentiating cells contains a small cytokine-like factor (AAV-induced factor [AIF]). The factor was characterized as peptide, which influences cell adhesion and appears to exist in forms of different activity connected by a trypsin-like cleavage site. It modulates cell growth in opposite ways: proliferation of tumor-derived cells is inhibited and fibroblasts are stimulated. The observations suggest that integration of AAV may result in cytokine induction and thus can indirectly affect growth of distant AAV-free target cells in a paracrine or endocrine manner. This is of interest with regard to antioncogenic properties of AAV and may also imply the possibility of AAV pathogenicity.