Integrated optical difference interferometer as immunosensor

✍ Scribed by Ch. Stamm; W. Lukosz

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 416 KB
• Volume: 31
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/0925-4005(96)80067-0

✦ Synopsis

We have used an integrated optical difference (or polarimetric) interferometer as an immunosensor. The goat anti-rabbit IgG antibodies are immobilized on a planar SiO2-TiO2 waveguide as receptor layer. The corresponding ligand, the antigen rabbit igG in the sample solution, is selectively detected in sub-nanomolar concentrations in less than 10 rain. The following technique for immobilizing the antibody leads to a stable receptor layer: the waveguide is first coated with avidin by adsorption, as avidin adheres well on it; in a second step, the biotinylated antibodies are bound irreversibly to the avidin layer by the strong avidin-biotin affinity binding.