Insulin uptake, localization and production in previously insulin treated and untreated Tetrahymena. Data on the mechanism of hormonal imprinting
✍ Scribed by G. Csaba; P. Kovács
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 486 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0263-6484
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✦ Synopsis
Confocal microscopic experiments demonstrate the presence of insulin in Tetrahymena\ observed also in earlier experiments[ However\ there is a broad spectrum of insulin!containing cells from the immunocytochemically insulin! free\ to the strongly antibody!reactive ones[ During 0 h of insulin treatment "imprinting# the cells gradually bind and take up insulin\ and the process is slow[ One minute after the start of treatment there is no di}erence in the number of insulin antibody!reactive cells and amount of insulin[ After 4 or 09 min the cells bind and contain more insulin and after 0 h most of the cells are densely packed with the insulin antibody!reactive material[ Insulin imprinting accelerates binding and uptake alike] 37 h after imprinting and 0 min after the start of the second treatment\ more insulin is present on the surface and inside the cells\ than after 09 min in the _rst!time treated cells[ Theoretically\ this e}ect of hormonal imprinting helps to maintain the species by facilitating molecular recognition and binding as well as uptake of useful molecules[ The experiments also support previous observations on the parallel receptor! evoking "strengthening# and hormone!producing e}ect of hormonal imprinting [ Copyright Þ 1999 John Wiley