✦ LIBER ✦

Insulin signaling: Tyrosine kinase activity in the crabChasmagnathus granulata gills

✍ Scribed by Kucharski, Luiz Carlos; Capp, Edison; Chitt�, Ana L�cia F.; Trapp, M�rcia; da Silva, Roselis S.M.; Marques, Maria

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 157 KB
Volume: 283
Category: Article
ISSN: 0022-104X
DOI: 10.1002/(sici)1097-010x(19990101)283:1<91::aid-jez10>3.0.co;2-f

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✦ Synopsis

Insulin-specific binding sites were recently shown in the gills of the estuarine crab Chasmagnathus granulata. In order to study the insulin receptor in these gills, its tyrosine kinase activity was evaluated in crude membranes and in isolated insulin receptor. The tyrosine kinase activity was determined by the insulin receptor capacity to autophosphorylate and phosphorylate exogenous substrate poly (Glu:Tyr 4:1) in the presence of bovine insulin (10 -7 M). When stimulated with insulin the 95 kDa β-subunit was phosphorylated and identified by autoradiography. The insulin receptor stimulated with insulin showed an 1.3-fold increase of [g 32 P]ATP incorporation to the exogenous substrate poly (Glu:Tyr 4:1). This increase was not observed when previously incubated with genistein. Further studies will be required to understand other steps of the insulin signaling chain in this organ.

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