Insulin-Like Synergistic Stimulation of DNA Synthesis in Swiss 3T3 Cells by the BSC-1 Cell-Derived Growth Inhibitor Related to Transforming Growth Factor Type β
✍ Scribed by Kenneth D. Brown and Robert W. Holley
- Book ID
- 123634178
- Publisher
- National Academy of Sciences
- Year
- 1987
- Tongue
- English
- Weight
- 955 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/29475
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Fibroblast Growth Factor (FGF) stimulates quiescent Swiss 3T3 cells to initiate DNA synthesis and divide. Cells begin to enter the S-phase after a lag of 13-15 hr, and the rate of initiation of DNA synthesis in the population can be quantified by a first order rate constant, k. A subsaturating conce
Swiss 3T3 mouse fibroblasts were exposed to 10 microM colchicine to disrupt microtubules, then stimulated with insulin-like growth factor-I. Immunoprecipitation experiments showed that insulin-like growth factor-I receptor and insulin receptor substrate-1 were tyrosine phosphorylated to the same ext