𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Insulin-like growth factor binding protein-3 is overexpressed in endothelial cells of mouse breast tumor vessels

✍ Scribed by Michael C. Schmid; Marco Bisoffi; Antoinette Wetterwald; Elsbeth Gautschi; George N. Thalmann; Stefania Mitola; Federico Bussolino; Marco G. Cecchini

Publisher
John Wiley and Sons
Year
2002
Tongue
French
Weight
326 KB
Volume
103
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Angiogenesis is a key process in a variety of human diseases, including cancer. The ability to target selectively the tumor vasculature is potentially useful for the diagnosis and treatment of cancer. Still, little information is available regarding markers that are restricted to the ECs of tumor vessels. cDNA array technology allows simultaneous analysis of relative expression levels of a broad spectrum of genes in 2 related cell populations. We used this technology with the aim of identifying markers specific for TECs. TECs were isolated by CD31‐mediated immunomagnetic separation from tumors induced by s.c. injection of NF9006 breast carcinoma cells into syngeneic mice. NECs were isolated from lactating mammary glands. The endothelial nature of isolated cells was confirmed by RT‐PCR using CD31‐specific primers and by uptake of DiI‐Ac‐LDL. Macrophage contamination in the EC isolations could be reasonably ruled out by assessing the expression of the macrophage marker c‐fms. ^32^P‐labeled cDNA probes generated by reverse transcription from total RNA were hybridized to mouse‐specific gene arrays. Several genes consistently showed differential expression between TECs and NECs. However, expression of only 1 of these genes, IGFBP‐3, was restricted exclusively to ECs. Semiquantitative RT‐PCR revealed 22‐ to 33‐fold differential expression of IGFBP‐3 in the TEC fraction. IGFBP‐3 was overexpressed by a factor of 5 in an additional mouse model of breast carcinoma induced by 4T1.2 tumor cells. These results indicate that IGFBP‐3 is a potential novel marker of angiogenesis. Elucidation of its role in tumor neovascularization may open the possibility of IGFBP‐3 as a therapeutic target for antiangiogenesis. © 2002 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Role of insulin-like growth factor bindi
Role of insulin-like growth factor binding protein-3 in breast cancer cell growth
✍ Lynette J. Schedlich; Lloyd D. Graham 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 165 KB 👁 1 views

The mitogenic effects of insulin-like growth factors (IGFs) are regulated by a family of insulin-like growth factor binding proteins (IGFBPs). One member of this family, IGFBP-3, mediates the growth-inhibitory and apoptosis-inducing effects of a number of growth factors and hormones such as transfor

Insulin-like growth factor binding prote
Insulin-like growth factor binding protein-3 (IGFBP-3) potentiates paclitaxel-induced apoptosis in human breast cancer cells
✍ C.A. Fowler; C.M. Perks; P.V. Newcomb; P.B. Savage; J.R. Farndon; J.M.P. Holly 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 145 KB 👁 2 views

Variability in response to chemotherapy is poorly understood. Paclitaxel-induced apoptosis was assessed in human Hs578T breast cancer cells, using the MTT assay, cell counting, morphological features and flow cytometry. Pre-dosing cells with non-glycosylated insulin-like growth factor binding protei

Insulin-like growth factor binding prote
Insulin-like growth factor binding protein (IGFBP)-5 is upregulated during both differentiation and apoptosis in primary cultures of mouse mammary epithelial cells
✍ Jennifer D. Lochrie; Kirsten Phillips; Elizabeth Tonner; David J. Flint; Gordon 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 306 KB 👁 2 views

## Abstract We have previously demonstrated that insulin‐like growth factor binding protein‐5 (IGFBP‐5) is upregulated following treatment of the mouse mammary epithelial cell line HC11 with lactogenic hormones (dexamethasone, insulin, and prolactin—DIP). In addition, we have also shown that IGFBP‐

Insulin-like growth factor (IGF) binding
Insulin-like growth factor (IGF) binding protein-3 regulation of IGF-I is altered in an acidic extracellular environment
✍ Kimberly E. Forsten; R. Michael Akers; James D. San Antonio 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB

## Abstract While extracellular acidification within solid tumors is well‐documented, how reduced pH impacts regulation of insulin‐like growth factor‐I (IGF‐I) has not been studied extensively. Because IGF‐I receptor binding is affected by IGF binding proteins (IGFBPs), we examined how pH impacted

Tumor necrosis factor-α–induced apoptosi
Tumor necrosis factor-α–induced apoptosis is associated with suppression of insulin-like growth factor binding protein-5 secretion in differentiating murine skeletal myoblasts
✍ Kate A. Meadows; Jeff M.P. Holly; Claire E.H. Stewart 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 264 KB 👁 1 views

Wasting of muscle and fat during cachexia exceeds that explained by reduced food intake alone. This wasting may result from an imbalanced cytokine environment, which could lead to increased protein catabolism. Supporting this, tumor necrosis factor-alpha (TNF-alpha) is raised in several animal model