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Insulin-induced rumplessness of chickens. II. Experiments with inactivated and reactivated insulin

✍ Scribed by Landauer, Walter ;Lang, Everett H.

Publisher: John Wiley and Sons
Year: 1946
Tongue: English
Weight: 475 KB
Volume: 101
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401010103

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✦ Synopsis

cross-over " against the original crystalline insulin and calculating the potency by relative drop (Hershey and Lacey, '36). A value of 26.5 units per milligram was found. The nitrogen content was 15.66%, giving an insulin nitrogen ratio of 1 unit C 0.00591 mg of nitrogen. A test for zinc by dithizone showed less than 5 y per 100 mg.

Active insulin (solutiota IX-56B)

One hundred milligrams of the stock insulin were dissolved in 25 cm3 of 0.276 phenol solution containing 0.8cma of 0.2N hydrochloric acid. The solution was filtered through a pyres

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