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Instability of in vitro resistance to imipenem in Pseudomonas aeruginosa

✍ Scribed by Ernst-Jürgen Wolter; Dr. Wolfgang Witte

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
305 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

In 3 from 19 clinical isolates of Pseudoinonas aeruginosa imipenem-resistant subpopulations could be detected in vitro. In comparison to their wild strains these imipenem-resistant cells were lacking an outer-membraneprotein of 50 kD. In the subpopulation derived from Pseudoinonas aeruginosa 76 resistance to imipenem was found to be instable. I t could be lost within a short period of time after subcultivation in the absence of imipenem. Cells with restored sensitivity to imipenem possess the outer membrane protein of 50 kD as thc wild strain does.

Imipenem, a representative of the carbapenem antibiotics, excerts a broad spectrum of antibacterial activity including P. aeruginosa (BAUERNFEJND 1985. The emergence of resistance has been observed in cause of the monotherapy of infections with P. aeruginosa . In vitro selection of imipenem-resistant parts of populations of P. aeruginosa has also been described (BUSCHER et al. 1987, CULLMANN e t a / . 1986). Wild isolates resistant to imipenem as well as resistant derivatives selected in virro show a diminished expression or the complete lack of an outer membrane protein (OMP) with a molecular mass of 49 kD (CULLMANN eta/. 1968) or 45-46 kD (BUSCHER et n/. 1987). The resistance is specific for imipenem and not affecting the action of other antibacterial agents (OPFERKUCH and CULLMANN 1987). In connection with experiments on selection of imipenem-resistant cells from clinical isolates of P. aeruginosa we found this resistance character to be not always stable.

This paper reports on the frequency of imipenem-resistant subpopulations among clinical isolates of P. aeruginosa, on the analysis of O M P in both clinical isolates and resistant derivatives and on the instability of the resistance phenotype.

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