✦ LIBER ✦

Insight into the Distribution of Molecular Weights and Higher-Order Structure of Hyaluronans and some β-(1→3)-Glucans by Size Exclusion Chromatography

✍ Scribed by Ladislav Šoltés; Danica Mislovičová; Bernard Sébille

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 626 KB
Volume: 10
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199603)10:2<53::aid-bmc552>3.0.co;2-5

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✦ Synopsis

The effects of high energy ultrasound and slightly raised temperature combined with the denaturing action of dimethylsulphoxide on the molecular weight and higher-order structure of hyaluronans and some &(1+ 3)-glucans were studied by means of size exclusion chromatography (SEC) technique. Some experimental conditions connected with the (bio-)polymer sample preparation prior to its SEC analysis are overviewed in the light of informational relevance of studies where the action of a physical and/or chemical agent changes the hydrodynamic size of the m omolecule.