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Insertion of MAP4-VP1 Peptide Into Lipid Monolayers and Bilayers

✍ Scribed by C. Mestres; I. Haro; F. Reig; M. A. Alsina

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
194 KB
Volume
11
Category
Article
ISSN
0269-3879

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✦ Synopsis

Myscibility of dipalmitoylphosphatidylcholine(DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role.

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