Insect resistance of transgenic plants that express modifiedBacillus thuringiensis cryIA(b)andcryICgenes: a resistance management strategy

✍ Scribed by Theo Salm; Dirk Bosch; Guy Honée; Lanxiang Feng; Ellie Munsterman; Petra Bakker; Willem J. Stiekema; Bert Visser

• Book ID: 104618066
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 697 KB
• Volume: 26
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00039519

✦ Synopsis

Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified crylA(b) and crylC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a crylC-crylA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.