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Inorganic nitrogen metabolism in two cellulose-degrading clostridia

โœ Scribed by Michael Bogdahn; Diethelm Kleiner


Book ID
104777862
Publisher
Springer
Year
1986
Tongue
English
Weight
267 KB
Volume
145
Category
Article
ISSN
0302-8933

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โœฆ Synopsis


Inorganic nitrogen metabolism in two cellulose degrading clostridia, the mesophile Clostridium cellobioparum and the thermophile Clostridium thermocellum was investigated. Both strains show acetylene reduction (i.e. possibly nitrogenase activity), contain glutamine synthetase, glutamate dehydrogenase and glutamate-dependent transaminases. C. ceIlobioparum additionally contains a NADHdependent glutamate synthase and a NH~-repressible glycine dehydrogenase (NADPH). Remarkably, acetylene reduction in C. thermocellum is not repressed by ammonium, casting doubt whether this activity is due to nitrogenase. The results are compared with the data from other saccharolytic clostridia.


๐Ÿ“œ SIMILAR VOLUMES


Regulatory aspects of inorganic nitrogen
โœ Kassem Alef; Diethelm Kleiner ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Springer ๐ŸŒ English โš– 295 KB

Regulatory aspects of the assimilation of inorganic nitrogen compounds (ammonia, nitrate, nitrogen) were studied in 12 strains belonging to the Rhodospirillaceae. All strains possessed an ammonium transport system, as demonstrated by ~4C-methylammonium uptake. This uptake showed saturation kinetics