Inorganic nitrogen metabolism in two cellulose-degrading clostridia
โ Scribed by Michael Bogdahn; Diethelm Kleiner
- Book ID
- 104777862
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 267 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
โฆ Synopsis
Inorganic nitrogen metabolism in two cellulose degrading clostridia, the mesophile Clostridium cellobioparum and the thermophile Clostridium thermocellum was investigated. Both strains show acetylene reduction (i.e. possibly nitrogenase activity), contain glutamine synthetase, glutamate dehydrogenase and glutamate-dependent transaminases. C. ceIlobioparum additionally contains a NADHdependent glutamate synthase and a NH~-repressible glycine dehydrogenase (NADPH). Remarkably, acetylene reduction in C. thermocellum is not repressed by ammonium, casting doubt whether this activity is due to nitrogenase. The results are compared with the data from other saccharolytic clostridia.
๐ SIMILAR VOLUMES
Regulatory aspects of the assimilation of inorganic nitrogen compounds (ammonia, nitrate, nitrogen) were studied in 12 strains belonging to the Rhodospirillaceae. All strains possessed an ammonium transport system, as demonstrated by ~4C-methylammonium uptake. This uptake showed saturation kinetics