Inorganic and organic selenium compound speciation with coupled HPLC–MW–HG–AFS

✍ Scribed by J. L. Gómez-Ariza; D. Sánchez-Rodas; E. Morales; O. Herrgott; I. L. Marr

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 64 KB
• Volume: 13
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(199910)13:10<783::aid-aoc907>3.0.co;2-7

✦ Synopsis

Coupling between high-performance liquid chromatography, microwave-induced reduction, hydride generation and atomic fluorescence spectrometry (HPLC-MW-HG-AFS) has been used for selenium speciation. The concentrations of a redox mixture (HCl solution of KBr and KBrO 3 aqueous solutions) used for both destruction of organic matter and reduction of the selenium species have been optimized, as well as the concentration of NaBH 4 in the hydride generation step. Chromatographic separation of selenocysteine, selenite and selenate took place in 15 min, employing a polymer-based strong anion-exchange column. Calibrations with correlation coefficients r b 0.998 were obtained for the three compounds at concentrations between 0.5 and 10 mg l À1 . Detection limits for selenite, selenocysteine (SeCys) and selenate were 0.2, 0.3 and 0.5 mg l À1 , respectively. The coupling has been tested with spiked water samples (tapwater and seawater), obtaining recoveries in the range 94-104%.