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Ink-native electrophoresis: An alternative to blue-native electrophoresis more suitable for in-gel detection of enzymatic activity

โœ Scribed by Kaneko, Keisuke; Sueyoshi, Noriyuki; Kameshita, Isamu; Ishida, Atsuhiko


Book ID
123143265
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
467 KB
Volume
440
Category
Article
ISSN
0003-2697

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โœ Jean Rivoal; Christopher R. Smith; Trevor F. Moraes; David H. Turpin; William C. ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 129 KB

We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m