Ink-Jet Microdispensing for the Formation of Gradients of Immobilised Enzyme Activity

✍ Scribed by Florin Turcu; Gerhard Hartwich; Dominik Schäfer; Wolfgang Schuhmann

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 197 KB
• Volume: 26
• Category: Article
• ISSN: 1022-1336
• DOI: 10.1002/marc.200400464

✦ Synopsis

Abstract

Summary: The integration of gradients of enzyme activity in microstructured biosensor arrays enables intrinsic on‐line quality control of biosensor performance. Multiple sensor elements with different compositions and hence varying responses for the same analyte are evaluated as a basis for improving data reliability. The formation of glucose oxidase/polymer microstructures using a piezo microdispenser and their examination by scanning electrochemical microscopy (SECM) are used to demonstrate the feasibility of this approach.

Optical microscope image of grids obtained by dispensing of 1 mg/mL GOx and 2 mg/mL Vinnapas® mixture.

magnified imageOptical microscope image of grids obtained by dispensing of 1 mg/mL GOx and 2 mg/mL Vinnapas® mixture.