Injectable rhBMP-2-loaded chitosan hydrogel composite: Osteoinduction at ectopic site and in segmental long bone defect
✍ Scribed by Ludmila Luca; Anne-Laure Rougemont; Beat H. Walpoth; Ludovic Boure; Andrea Tami; James M. Anderson; Olivier Jordan; Robert Gurny
- Book ID
- 102873701
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 953 KB
- Volume
- 96A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
Carriers for bone morphogenetic protein‐2 (BMP‐2) used in clinical practice still suffer from limitations such as insufficient protein retention. In addition, there is a clinical need for injectable carriers. The main objective of this study was to assess bone forming ability of rhBMP‐2 combined either with chitosan hydrogel (rhBMP‐2/CH) or chitosan hydrogel containing β‐tricalcium phosphate (β‐TCP) (rhBMP‐2/CH/TCP). Formulations were first compared in a rat ectopic intramuscular bone formation model, and the optimal formulation was further evaluated in healing of 15‐mm critical size defect in the radius of a rabbit. Three weeks after injection ectopically formed bone was analyzed by microcomputerized tomography (micro‐CT) and histology. Significantly higher (4.7‐fold) mineralized bone formation was observed in the rhBMP‐2/CH/TCP group compared to rhBMP‐2/CH group. In a pilot study, defect in a rabbit radius treated with rhBMP‐2/CH/TCP showed incomplete regeneration at 8 weeks with composite leakage from the defect, indicating the need for formulation refinement when segmental defect repair is foreseen. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2010.