Initiation, Release, and Reinitiation of RNA Chains by Bacteriophage-T3-Induced Polymerase from T3 DNA Templates
β Scribed by Umadas Maitra and Henry H. Huang
- Book ID
- 123650483
- Publisher
- National Academy of Sciences
- Year
- 1972
- Tongue
- English
- Weight
- 811 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/60979
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π SIMILAR VOLUMES
## Abstract Dimethylsulfoxide (DMSO)Abbreviations used: Dimethylsulfoxide DMSO, polyvinyl sulfate PVS, nucleoside triphosphate NTP up to 25% (v/v) does not cause irreversible alterations of T3 DNA at 42.5Β°C as assayed by transcription with T3βspecific RNA polymerase. The optimal temperature for th
## Abstract Bacteriophage T3βinduced RNA polymerase is rapidly inactivated at 42Β°C. Addition of T3 DNA delays this process for 30 s and reduces the rate with which the enzyme activity is lsot indicating that a labile binary complex between T3 DNA and polymerase must have been formed. The ternary co