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Initiation of the polypeptide chain in the rII B cistron of bacteriophage T4

โœ Scribed by Sarabhai, Anand; Lamfrom, Hildegard


Book ID
122441752
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
363 KB
Volume
52
Category
Article
ISSN
0022-2836

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In this report the herefore uncharacterized mutants at the beginning of the rIIB cistron of bacteriophage T4 have been connected to the main part of the map and have been shown not to be in any way unusual. The temperature sensitive mutant HD263 appears to be the earliest mutant in the cistron.

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The EcoRI digestion products of phage T4 DNA have been examined using a phage DNA transformation assay. A 2.6 X 10(6) Dalton fragment was found to contain the rII genes. This fragment was purified and then treated with HindIII endonuclease. The cleavage products were ligated to the vector plasmid pB