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Initiation of DNA synthesis in a high molecular weight fraction of Xenopus egg extract.

✍ Scribed by Koji Okuhara; Masaki Shioda; Koichiro Shiokawa; Kimiko Murakami-Murofushi; Yousuke Seyama; Hiromu Murofushi

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
402 KB
Volume
17
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

Xenopus egg extract was fractionated by gel‐filtration column chromatography and DNA synthetic activity was examined using double‐stranded DNA as a template. The major activity eluted had an apparent molecular mass of about 300 kDa. Product analyses showed that de novo DNA synthesis occurs with formation of replication bubbles, thereby suggesting that this fraction catalyzes the initiation of DNA replication. Activities of DNA polymerase α‐primase and DNA helicase overlapped with the DNA synthetic activity, but the elution profiles of the enzymes differed from that of the DNA synthetic activity. Therefore, this 300‐kDa fraction may contain a component which differs from the above enzymes yet is essential for initiation of DNA replication.

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