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Initiation of DNA Replication Requires the RECQL4 Protein Mutated in Rothmund-Thomson Syndrome

✍ Scribed by Mahesh N. Sangrithi; Juan A. Bernal; Mark Madine; Anna Philpott; Joon Lee; William G. Dunphy; Ashok R. Venkitaraman


Book ID
116352650
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
763 KB
Volume
121
Category
Article
ISSN
0092-8674

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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins