Initiation of DNA replication in Δcya mutants of Escherichia coli K12
✍ Scribed by Ahmed Landoulsi; Masamichi Kohiyama
- Book ID
- 117457181
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 261 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0300-9084
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When E. coli F+ cells carrying the dna-167 or dnaC2 mutation, which causes the temperature-sensitive initiation of DNA replication, are exposed to a non-permissive temperature to stop the replication of chromosome and F factor, and then transferred back to a permissive temperature with the addition
Temperature-sensitive mutants defective in the initiation of DNA replication are exposed to a non-permissive temperature to complete already initiated replication, and are transferred back to a permissive temperature. DNA synthesis can resume in the presence of rifampicin or rifampicin plus chloramp
It has been found that strains carrying mutations in the dnaA gene are unusually sensitive to COU, NAL or NOV, which are known to inhibit DNA gyrase activities. The delay in the initiation of chromosome replication after COU treatment has been observed in cells with chromosomes synchronized by amino