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Initiation of cleavage inOryzias latipes eggs injected with centrioles from sea urchin spermatozoa

✍ Scribed by Ohta, T. ;Iwamatsu, T.

Book ID
102891521
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
620 KB
Volume
214
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Cleavage‐initiating activity of various biological materials was tested by microinjection into unfertilized eggs of Oryzias latipes. Injection of intact sea urchin spermatozoa, their flagellum‐ and centriole‐fractions induced cleavage of unfertilized eggs. On the contrary, injection of the suspension medium alone did not induce cleavage. In the process of a ster formation elicited by injection, fibrous structures appeared first within a dispersing mass of the injected fraction, and they then developed into a few large or many small astral structures. Eggs which cleaved within 2 hr after injection displayed only a few large asters within a thickened blastodisc. In the non‐cleaved eggs, only many small asters appeared throughout the blastodisc.

These experiments indicated that the isolated centrioles from sea urchin spermatozoa can induce aster formation and cleavage in the fish.

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Morphological observations on cleavage of the egg of the medaka,Oryzias latipes, following injection with flagellar microtubules of sea urchin spermatozoa
✍ Ohta, Tadayuki ;Iwamatsu, Takashi 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 880 KB

## Abstract Mature unfertilized eggs of the medaka, __Oryzias latipes__, were injected with sperm flagellar microtubules of the sea urchin, __Hemicentrotus pulcherrimus__, and were observed with reference to the process of early development. Groups of injected eggs, most of which initiated cleavage