Initiation methionine of recombinant interleukin-2 is completely processed in vivo by replacement of the second proline residue

In-vivo processing of the initiator meth

In-vivo processing of the initiator methionine from recombinant methionyl human interleukin-6 synthesized inEscherichia colioverproducing aminopeptidase-P

✍ Hisashi Yasueda; Yoshimi Kikuchi; Hiroyuki Kojima; Kazuo Nagase 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 665 KB

Human interleukin 6 (hIL-6) overproduced in Escherichia coli HB101 was found to partially retain the initiator methionine (Met) residue (Met-hIL-6). In order to remove the residual N-terminal Met in vivo, an attempt was made to express hIL-6 in aminopeptidase-P (Ap-P)-hyperproducing strains, since t