Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase
β Scribed by Ha, Taekjip; Rasnik, Ivan; Cheng, Wei; Babcock, Hazen P.; Gauss, George H.; Lohman, Timothy M.; Chu, Steven
- Book ID
- 109890561
- Publisher
- Nature Publishing Group
- Year
- 2002
- Tongue
- English
- Weight
- 228 KB
- Volume
- 419
- Category
- Article
- ISSN
- 0028-0836
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins
The synthesis and action of the dnaA product with respect to DNA initiation and the synthesis of DNA-binding proteins in Escheriehia coli was examined. Results indicate that when dnaA product is irreversibly denatured and must be synthesized before initiation can occur, its synthesis and action appe
The capacity for initiation and subsequent chain elongation was examined in several DNA temperature sensitive mutants of Escherichia coli after the mutants had been held at nonpermissive temperature for approximately 1.5 generation equivalents and then returned to permissive temperature in the prese
This paper deals with. the conditions that are necessary for the acquisition and expression of initiation potential in dnaA temperature sensitive mutants after they have been held for peri: ods of time at nonpermissive temperature and then returned to permissive temperature in the presence of chlora