Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries ofVitis longii‘Microsperma’

✍ Scribed by D. J. Gray; J. A. Mortensen

• Book ID: 104621948
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 978 KB
• Volume: 9
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00046081

✦ Synopsis

Anthers and ovaries of Vitis longii 'Microsperma' produced embryogenic callus when cultured on solidified Murashige and Skoog medium with 5/~M 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 #M benzyladenine (BA). The initial callus was short-lived. However, long-term embryogenesis from callus was maintained through serial transfers by careful selection of clustered embryos with subtending callus. Alternatively, long term culture maintenance was through secondary embryogenesis which occurred directly from previously formed embryos on medium lacking growth regulators. Somatic embryos were white, exhibited frequent pluricotyly and tended to be larger than zygotic embryos. Histology of embryogenic callus demonstrated the presence of lipidlike substances and abundant starch. Somatic embryos were attached to callus by narrow to wide suspensor-like structures and possessed typical epidermal, cortical, and vascular tissue. Embryo cells contained abundant lipid-like accumulations but no starch. Embryos germinated when placed on medium containing 1 #M BA and produced plants of normal appearance.