Initial results of endothelial cell seeding following argon laser carotid endarterectomy

✍ Scribed by Alan P. Sawchuk; Malcolm B. Herring; Michael C. Dalsing

• Book ID: 102936279
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 857 KB
• Volume: 12
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.1900120602

✦ Synopsis

This study evaluates the initial results of endothelial cell (EC) seeding following argon laser carotid endarterectomy. Venous endothelial cells were harvested from 12 dogs and cultured. A laser endarterectomy was performed on both carotids of each dog. One side was seeded with endothelial cells. Six dogs had both carotids harvested 1 hour after restoring blood flow. The others were harvested in 24 hours. The percentage of lumen covered with EC was evaluated by scanning electron microscopy. At 1 hour, the seeded arteries demonstrated 35 -t 3 percent EC coverage, whereas the unseeded arteries had no EC coverage (P = 0.0002). At 24 hours, the seeded arteries had 58 rt 15 percent EC coverage, whereas the unseeded arteries had no coverage (P = 0.01). Significant gross thrombus developed only in unseeded arteries (P = 0.0471, two of which were occluded at 24 hours. EC seeding is beneficial following argon laser carotid endarterectomy resulting in improved patency and less surface thrombogenicty. o 1992 Wiley-Liss, Inc.