✦ LIBER ✦

Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation

✍ Scribed by Jung-Hoe Kim; Wei-Shou Hu

Publisher: Springer
Year: 1989
Tongue: English
Weight: 761 KB
Volume: 2
Category: Article
ISSN: 0920-9069
DOI: 10.1007/bf00386146

No coin nor oath required. For personal study only.

✦ Synopsis

Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.