Initial Characterization of a Temperature Sensitive Mutant of Monkey Kidney Cell
โ Scribed by NAHA, P. M.
- Book ID
- 109675574
- Publisher
- Nature Publishing Group
- Year
- 1970
- Tongue
- English
- Weight
- 471 KB
- Volume
- 228
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/228166a0
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract We have analyzed ongoing DNA replication in __ts__ BNโ2, a __dna__^โ^ mutant of BHKโ21 cells (Nishimoto et al. '78). At the nonโpermissive temperature of 39.5ยฐC, inhibition of ^3^Hโthymidine into acidโprecipitable material begins 1 to 2 h after the cells are released from a block at the
## Abstract A temperatureโsensitive growth mutant derived from the BHK 21 cell Line, ts AF8, was found to have greatly reduced DNA synthesis at the nonpermissive temperature. This reduction is mainly due to a decrease in the frequency of cells synthesizing DNA. Upon shift up, ts AF8 becomes blocked