Inhibitory effects of chlorophyllin on micronucleus formation induced by ethyl carbamate and its proximate and ultimate carcinogenic forms in mouse peripheral reticulocytes
✍ Scribed by Seong Gon Kim; Young-Joon Surh; James A. Miller
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 59 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Methods
EC and B[a]P were supplied by Sigma Chemical Co. (St. Louis, MO). VC and VCO were synthesized as described previously [Park et al., 1990]. CHL was purchased from Aldrich Chemical Co. (Milwaukee, WI). Trioctanoin was a product of the Pfaltz and Bauer Inc. (Stamford, CT). Male C3H/HeJ and female C57BL/6J mice from the Jackson Laboratory (Bar Harbor, ME) were bred in our laboratory to give B6C3F1 offspring. Male B6C3F1 mice (8 -10 weeks of age) were gavaged twice with CHL (100 mg/kg) 1 hr and immediately before a single intraperitoneal injection of EC (500 mg/kg), VC (25 mg/kg), VCO (10 mg/kg), or B[a]P (200 mg/kg). EC and VC were dissolved in phosphate-buffered saline (pH 7.4), whereas VCO and B[a]P were dissolved in trioctanoin immediately before administration. Peripheral blood was collected from the tail vein just before or 24, 48, and 72 hr after the clastogen administration and placed on an acridine orange-coated glass slide. The frequencies of micronucleated reticulocytes were scored according to the method of Hayashi et al. [1990].