Inhibitory effect of Pruni Cortex extract and its component, octacosyl ferulate, on tumour promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin
✍ Scribed by Ken Yasukawa; Saša M. Dimitrijevic; Fred J. Evans; Shigeru Kawabata; Michio Takido
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 338 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0951-418X
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✦ Synopsis
The methanol extract of Pruni Cortex inhibited tumour promotion by 12-O-tetradecanoylphorbol-13acetate (TPA) in 7,12-dimethylbenz[a]anthracene (DMBA)-initiated mice. The active component was isolated from the methanol extract of Pruni Cortex. The active compound was characterized as octacosyl ferulate by physical and chemical analysis. Octacosyl ferulate markedly inhibited the inflammatory activity induced by TPA in mice. The 50% inhibitory dose of octacosyl ferulate is 0.7 mg/ear on TPA-induced inflammation. Furthermore, octacosyl ferulate suppressed markedly the tumour-promoting effect of TPA on skin tumour formation following initiation with DMBA. Since protein kinase C (PK-C) was shown to be an intracellular target of TPA, effects of octacosyl ferulate on the activity of this enzyme were investigated. Octacosyl ferulate also inhibited the phosphorylation of histon III-S by PK-C in a concentration dependent manner.