Inhibitory effect of ethanol on growth and solute accumulation bySaccharomyces cerevisiaeas affected by plasma-membrane lipid composition
✍ Scribed by D. Susan Thomas; A. H. Rose
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 558 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Incorporation of ethanol (1.0 or 1.25 M) into exponential-phase cultures of Saccharomyces cerevisiae N C Y C 366 growing anaerobically in a medium supplemented with ergosterol and an unsaturated fatty acid caused a retardation in growth rate, which was greater when the medium contained oleic rather than linoleic acid. Ethanol incorporation led to an immediate drop in growth rate, and ethanol-containing cultures grew at the slower rate for at least 10h. Incorporation of ethanol (0.5 M) into buffered (pH 4.5)
glucosamine, L-[U-14C] lysine or arginine, or KH232po4 lowered the rate of solute accumulation by cells. Rates of accumulation of glucose, lysine and arginine were retarded to a greater extent when cells had been grown in the presence of oleic rather than linoleic acid. This difference was not observed with accumulation of phosphate. Ethanol was extracted from exponential-phase cells by four different methods. Cells grown in the presence oflinoleic acid contained a slightly, but consistently, lower concentration of ethanol than cells grown in oleic acid-containing medium. The ethanol concentration in cells was 5 -7 times greater than that in the cell-free medium.