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Inhibition of vaccinia RNA guanine 7-methyltransferase by compounds designed as multisubstrate adducts

✍ Scribed by Eric Benghiat; Peter A. Crooks; Raymond Goodwin; Fritz Rottman


Book ID
102405789
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
445 KB
Volume
75
Category
Article
ISSN
0022-3549

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✦ Synopsis


Several potential inhibitors of mRNA guanine 7-methyltransferase, which were designed from mechanism-based considerations, were evaluated against the vaccinia virus capping enzyme complex. Of the compounds tested, 5'-deoxy-5'[6-(2-aminopyrrolo[2,3-d]-pyrimidine-4-one) methylthio]adenosine (9) had good selective inhibitory activity against vaccinia mRNA guanine 7-methyltransferase, exhibiting an IC50 of 9.2 X 10(-5) M. Structure-activity considerations suggest that specific inhibition of RNA methyltransferases by low molecular weight multisubstrate adduct inhibitors may be achievable.