An improved method for isolation of mito
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Jane A. Idell-Wenger; Lee W. Grotyohann; James R. Neely
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Article
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1982
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Elsevier Science
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English
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An improved procedure for the isolation of mitochondria in high yields from normal and oxygen-deficient myocardium is described. The heart muscle is digested with Nagarse and homogenized simultaneously using a Polytron tissue homogenizer. Mitochondria are isolated by differential centrifugation, and