Inhibition of the system luminol-H2O2-Fe(CN)63− chemiluminescence by the Mn(II) indirect determination of isoniazid in a pharmaceutical formulation
✍ Scribed by Alapont, A. Gregorio ;Giménez, E. Aurechia ;Zamora, L. Lahuerta ;Calatayud, J. Martínez
- Publisher
- John Wiley and Sons
- Year
- 1998
- Weight
- 118 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate (III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied to determination of the drug in a pharmaceutical formulation.