Inhibition of the RNA dependent DNA polymerase and the malignant transforming ability of Rous sarcoma virus by thiosemicarbazone-transition metal complexes
✍ Scribed by William C. Kaska; Carl Carrano; Joseph Michalowski; Jean Jackson; Warren Levinson
- Publisher
- Elsevier Science
- Year
- 1978
- Weight
- 600 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0006-3061
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✦ Synopsis
The half-lives of liver and kidney metallothionein of rats fed diets containing either 2,000 ppm Zn or LOO ppm Cd were determined by a single injection of "C-cystine. This protein was purified by Sephadex G-75 and DEAR cehulose column chromatography. Half-lives of liver metahothionein in rats fed the basal (18 ppm Zn), high Zn (2,000 ppm Zn), h&h Zn then basal, and high Cd (100 ppm) diet were, respectively, 3.4,2.7 1.3, and 3.3 days, and that of kidney metallotbionein were, respectively, 2.0. 2.2. 1.4, and 3.6 days.
Thus, Cd-thionein has a longer half-life than Zn-thionein in both liver and lcidney. The major species of metallothionein, which were separated on DEAE cellulose, had similar half-lives. Metallothionein, a protein having high content of metals and relatively short half-life, is possibly involved in cellular regulatory function.