A 186-base pair fragment of ors8, a mammalian autonomously replicating DNA sequence isolated by extrusion of nascent monkey DNA in early S phase, has previously been identified as the minimal sequence required for replication function in vitro and in vivo. This 186-base pair fragment contains, among
Inhibition of the In Vitro Replication of DNA by an Aptamer–Protein Complex in an Autonomous DNA Machine
✍ Scribed by Changfeng Zhu; Yanqin Wen; Di Li; Lihua Wang; Shiping Song; Chunhai Fan; Itamar Willner
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 379 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0947-6539
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
We have shown that antisense oligonucleotides can be targeted to hepatitis B virus (HBV)-infected cells, resulting in specific inhibition of viral protein synthesis and replication in vitro. The targeting system was based on the internalization of DNA complexes by highly selective receptors for gala
## Abstract __SELEX was used to create an RNA aptamer targeted to protein tyrosine phosphatase 1B (PTP1B), an enzyme implicated in type 2 diabetes, breast cancer and obesity. We found an aptamer that strongly inhibits PTP1B in vitro with a__ K__~i~ of less than 600 pM. This slow‐binding, high‐affin