Inhibition of rat peroxisomal palmitoyl-CoA ligase by xenobiotic carboxylic acids
โ Scribed by Benjamin J. Roberts; Kathleen M. Knights
- Book ID
- 115782562
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 885 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0006-2952
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๐ SIMILAR VOLUMES
A radiolabeled ATP assay was developed for measuring carboxylic acid:CoA ligase activity. The assay was designed to measure the formation of [gamma-33P]pyrophosphate from [gamma-33P]ATP in the course of the reaction. The assay was linear with protein concentration, and rates as low as 1 pmol/min wer
A mitochondria1 freezetthaw lysate was fractionated on a DEAE-cellulose column into four distinct acyl-CoA ligase fractions. First to elute was a 50 kDa short-chain ligase that activated only short-chain fatty acids. Next to elute were three ligases that had activity toward both medium-chain fatty a