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Inhibition of rat cervical epithelial cell growth by heparin and its reversal by EGF

✍ Scribed by Thomas C. Wright Jr.; Theresa V. Johnstone; John J. Castellot; Morris J. Karnovsky

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 970 KB
Volume: 125
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041250320

No coin nor oath required. For personal study only.

✦ Synopsis

The effects of heparin on the in vitro growth of rat cervical epithelial cells were examined. Heparin was found to inhibit in a dose dependent fashion the log-phase growth of rat cervical epithelial cells (RCEC) grown in the absence of medium supplements. An inhibition of growth is observed at concentrations as low as 500 ng/ml and 50% inhibition of growth occurs at a concentration of 5 pg/mI. The growth inhibitory activity of heparin is independent of anticoagulant activity since three separate non-anticoagulant preparations of heparin all inhibit growth. Other glycosaminoglycans including chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, hyaluronic acid, and keratin sulfate do not inhibit t h e growth of rat cervical epithelial cells. The ability of heparin to inhibit the log-phase growth of rat cervical epithelial cells is dependent on the composition of the medium in which the cells are grown. The addition of >, 7.5 ng/ml epidermal growth factor to epithelial cultures blocks the growth inhibitory activity of heparin. These results suggest that components of t h e extracellular matrix modulate the growth responses of epithelial cells and may be important in regulating cellular proliferation in normal and pathological states.

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