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Inhibition of protein kinase C and proto-oncogene expression by crocetin in NIH/3T3 cells

✍ Scribed by Chau-Jong Wang; Tzu-Chun Cheng; Jer-Yuh Liu; Fen-Pi Chou; Min-Liang Kuo; Jen-Kun Lin

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
849 KB
Volume
17
Category
Article
ISSN
0899-1987

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✦ Synopsis

Crocetin, a carotenoid isolated from the seeds of Gardeniajasminoides, was found to be a potent inhibitor of tumor promotion induced by 12-0-tetradecanoy1phorbo1-13-acetate (TPA) in mouse skin. When mouse fibroblast NIH/3T3 cells were treated with TPA alone, protein kinase C (PKC) translocated from the cytosolic fraction to the particulate fraction. Pretreatment with 60 and 120 pM crocetin for 15 min inhibited the TPAinduced PKC activity in the particulate fraction by 50% and 66%, respectively, but did not affect the level of PKC protein. Crocetin also reduced the level of TPA-stimulated phosphorylation of cellular proteins. Cells pretreated with crocetin (1 20 pM) had 55% less PKC [3H]phorbol dibutyrate-binding capacity. Suppression of TPA (100 ng/mL)-induced c-jun and c-fos gene expression was also observed in the mouse fibroblast cells pretreated with crocetin (30,60, and 120pM). Our results provided a basis for understanding the inhibitory effect of crocetin on TPA-mediated tumor promotion.

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