Inhibition of phosphatidylcholine-specific phospholipase C prevents bone marrow stromal cell senescence in vitro

✍ Scribed by Chunhui Sun; Nan Wang; Jie Huang; Jie Xin; Fen Peng; Yinshi Ren; Shangli Zhang; Junying Miao

• Book ID: 102301569
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 421 KB
• Volume: 108
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.22282

✦ Synopsis

Abstract

Bone marrow stromal cells (BMSCs) can proliferate in vitro and can be transplanted for treating many kinds of diseases. However, BMSCs become senescent with long‐term culture, which inhibits their application. To understand the mechanism underlying the senescence, we investigated the activity of phosphatidylcholine‐specific phospholipase C (PC‐PLC) and levels of integrin β4, caveolin‐1 and ROS with BMSC senescence. The activity of PC‐PLC and levels of integrin β4, caveolin‐1 and ROS increased greatly during cell senescence. Selective inhibition of increased PC‐PLC activity with D609 significantly decreased the number of senescence‐associated beta galactosidase positive cells in BMSCs. Furthermore, D609 restored proliferation of BMSCs and their differentiation into adipocytes. Moreover, D609 suppressed the elevated levels of integrin β4, caveolin‐1 and ROS. The data suggest that PC‐PLC is involved in senescence of BMSCs, and its function is associated with integrin β4, caveolin‐1 and ROS. J. Cell. Biochem. 108: 519–528, 2009. © 2009 Wiley‐Liss, Inc.