Inhibition of ornithine decarboxylase activity and cell growth by diamines: A comparison between the effects of two homologs, 1,3-diaminopropane and 1,4-diaminobutane (putrescine)
✍ Scribed by Margareta Linden; Stina M. Oredsson; Siw Anehus; Olle Heby
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 500 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
The ornithine decarboxylase (ODC) activity of Ehrlich ascites tumor cells was almost completely inhibited by treatment with either putrescine (10 mM) or 1,3diaminopropane (5 mM). 1,3-Diaminopropane treatment eradicated the cellular content of putrescine and reduced that of spermidine and spermine. Putrescine treatment caused a dramatic increase in cellular putrescine content and a temporary decrease in spermidine and spermine content. Despite the fact that 1,3-diaminopropane and putrescine inhibited the ODC activity more effectively than did a- difluoromethylornithine (DFMO), an enzyme-activated irreversible inhibitor of ODC, they were considerably less antiproliferative in action. However, as compared to DFMO the diamines were less effective in reducing the total polyamine (putrescine + spermidine + spermine) content of the cells.