Inhibition of mammalian target of rapamycin signaling potentiates the effects of all-trans retinoic acid to induce growth arrest and differentiation of human acute myelogenous leukemia cells
✍ Scribed by Chie Nishioka; Takayuki Ikezoe; Jing Yang; Sigal Gery; H. Phillip Koeffler; Akihito Yokoyama
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- French
- Weight
- 828 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Our study explored the drug interaction of all‐__tran__s retinoic acid (ATRA) and RAD001 (everolimus), the inhibitor of mammalian target of rapamycin complex 1 (mTORC1), in acute myelogenous leukemia (AML) NB4 and HL60 cells. RAD001 (10 nM) significantly enhanced the ATRA‐induced growth arrest and differentiation of these cells, as measured by colony‐forming assay and cell cycle analysis, and expression of CD11b cell surface antigen and nitroblue tetrazolium reduction, respectively. ATRA (0.1–1 μM) upregulated levels of RTP801, a negative regulator of mTORC1, and inhibited mTORC1 signaling as assessed by measurement of the levels of p‐p70S6K and p‐4E‐BP1 in HL60 and NB4 cells. ATRA (0.1–1 μM) in combination with RAD001 (10 nM) strikingly downregulated the levels of p‐70S6K and p‐4E‐BP1 without affecting the total amount of these proteins. Notably, RAD001 (10 nM) significantly augmented ATRA‐induced expression of CCAAT/enhancer‐binding protein ε (C/EBPε) and p27^kip1^ and downregulated levels of c‐Myc in these cells. Furthermore, RAD001 (5 mg/kg) enhanced the ability of ATRA (10 mg/kg) to inhibit the proliferation of HL60 cells growing as tumor xenografts in immune‐deficient nude mice. Taken together, concomitant blockade of the RA and mTORC1 signaling may be a promising treatment strategy for individuals with AML. © 2009 UICC