Inhibition of luminol-dependent luminescence and simultaneous generation of native luminescence of activated human polymorphonuclear leukocytes by addition of albumin

✍ Scribed by Roschger, Paul ;Graninger, Wolfgang ;Klima, Herbert

• Publisher: John Wiley and Sons
• Year: 1988
• Weight: 524 KB
• Volume: 2
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170020103

✦ Synopsis

Luminol-dependent luminescence (LDL) and luminol-independent, native luminescence (NL) of polymorphonuclear leukocytes were investigated with respect t o the effects generated by the addition of albumin t o the reaction medium. The cells were activated: (1) by simple surface attachment t o a hydrophilic plastic, (2) by opsonized zymosan, (3) by phorbol myristate acetate, (4) by formylmethionyl-leucyl-phenylalaline. Both kinds of emissions were recorded simultaneously using a method of spectral discrimination. The addition of albumin resulted in an inhibition of LDL, which coincided with a generation of NL. The extent of the inhibition of LDL depended on the type of stimulus used. Maximum inhibition occurred with cells activated by attachment t o plastic surfaces and minimum inhibition was observed with cells stimulated by opsonized zymosan. Different contributions of extracellularly released reactive oxygen-species may be responsible for this. It appears possible t o discriminate between intra-and extracellular sites of oxygen-metabolites production using albumin simultaneously as extracellular quencher of LDL and as luminescent probe for NL.