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Inhibition of lipase-catalyzed hydrolysis of sunflower oil in AOT/isooctane reversed micelles

✍ Scribed by Q -L. Chang; J -Y. Chen


Publisher
Springer
Year
1995
Tongue
English
Weight
308 KB
Volume
13
Category
Article
ISSN
1615-7605

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✦ Synopsis


The hydrolysis of sunflower oil using Candida cylindracea lipase in reversed micelles of AOT/isooctane was investigated. The inhibition caused by substrate and hydrolysis products has been found in the process of reaction. It was revealed that the extent of inhibition caused by oleic acid was higher than that caused by glycerol, and was much more serious in the case of the mixture of hydrolysis products. Moreover, with the initial addition of glycerol into the reaction mixture, the stability of lipase could be increased during the hydrolysis of sunflower oil in reversed micelles.


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## Abstract Candida rugosa lipase has been used to investigate the hydrolysis of high concentration olive oil in the AOT–isooctane reversed micellar system at __W__~__o__~ = 10, pH 7.1, and 37Β°C. Results from this work show the hydrolytic reaction obeys Michaelis–Menten kinetics up to the initial s