Inhibition of lactate transport in Ehrlich ascites tumor cells and human erythrocytes by a synthetic anhydride of lactic acid
β Scribed by Johnson, John H.; Belt, Judith A.; Dubinsky, William P.; Zimniak, Andrzej; Racker, Efraim
- Book ID
- 126489150
- Publisher
- American Chemical Society
- Year
- 1980
- Tongue
- English
- Weight
- 513 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0006-2960
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## Abstract The effects of the nonpenetrating amino reactive reagent 4βacetamidoβ4β²βisothiocyanoβstilbeneβ2β2β²βdilsulfonic acid (SITS) on anion transport (sulfate, chloride, and inorganic phosphate) were investigated in Ehrlich ascites tumor cells. Short time exposure to SITS produces a reversible
Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o